Sequencing

Recombinant Dna isolation, cloning and sequencing
Bst-catalyzed radiolabeled DNA sequencing, Radiolabeled sequencing gel preparation, loading, and electrophoresis, Taq-polymerase
catalyzed cycle sequencing using fluorescent-labeled dye primers, Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled
dye terminator reactions, Terminator Reaction Clean-Up via Centri-Sep Columns, Terminator Reaction Clean-Up via Sephadex G-50 Filled
Microtiter Format Filter Plates, Sequenase[TM] catalyzed sequencing with dye-labeled terminators, Fluorescent-labeled sequencing gel
preparation, pre-electrophoresis, sample loading, electrophoresis, data collection, and analysis on the ABI 373A DNA sequencer,
Double-stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers, cDNA sequencing based on PCR
and random shotgun cloning.

Sequencing protocol (Big Dye Terminator) 
Amplify the gene, purify tha sequencing template, run the sequencing reaction, purify the sequencing product, dry/submit the sample.